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|Column||Raptor ARC-18 (cat.# 9314A65)|
|Dimensions:||150 mm x 4.6 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor ARC-18 EXP guard column cartridge 2.7 µm (cat.# 9314A0250)|
|Inj. Vol.:||5 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Sample Preparation||20 mg of concentrate was weighed into a 50 mL centrifuge tube. 10 mL of ethanol was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.|