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|Column||Raptor ARC-18 (cat.# 931421E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||1.8 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter (cat.# 25809)|
|Inj. Vol.:||2 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Notes||1 g of ground hard candy was weighed into a 50 mL centrifuge tube. 5 mL of water was added and the sample was vortexed 30 seconds until the candy completely dissolved. Total volume was brought to 40 mL with IPA and vortexed for an additional 30 seconds. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.|