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|Column||Raptor ARC-18 (cat.# 931421E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||1.8 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter (cat.# 25809)|
|Inj. Vol.:||2 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Sample Preparation||20 mg of concentrate was weighed into a 50 mL centrifuge tube. 10 mL of ethanol was added prior to vortexing (5 seconds) and sonicating (5 minutes) over a total of three cycles. The sample was then centrifuged for 5 minutes at 3000 rpm. The supernatant was diluted 50-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.|
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