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Cannabis Chocolate Sample on Raptor ARC-18 1.8 μm by UHPLC-UV

ColumnRaptor ARC-18 (cat.# 931421E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:1.8 µm
Pore Size:90 Å
Guard Column:UltraShield UHPLC precolumn filter (cat.# 25809)
Temp.:30 °C
Diluent:25:75 Water:methanol
Inj. Vol.:2 µL
Mobile Phase
A:Water, 5 mM ammonium formate, 0.1% formic acid
B:Acetonitrile, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/Vis @ 228 nm
Sample Preparation2 g of chocolate was weighed into a 50 mL centrifuge tube. The sample was brought to a total volume of 40 mL with IPA. The sample was then sonicated at 40 °C for 5 minutes followed by gentle mixing by hand. This was repeated for an additional two cycles. The sample was stored in a -20 °C freezer for 30 minutes to allow the lipids to precipitate. The sample was then vortexed for 5 seconds followed by centrifugation at 3000 rpm for 5 minutes. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.