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Peaks | tR (min) | |
---|---|---|
1. | Δ9-Tetrahydrocannabinol (Δ9-THC) | 2.49 |
Column | Raptor ARC-18 (cat.# 931421E) | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Dimensions: | 100 mm x 3.0 mm ID | ||||||||||||
Particle Size: | 1.8 µm | ||||||||||||
Pore Size: | 90 Å | ||||||||||||
Guard Column: | UltraShield UHPLC precolumn filter (cat.# 25809) | ||||||||||||
Temp.: | 30 °C | ||||||||||||
Standard/Sample | |||||||||||||
Diluent: | 25:75 Water:methanol | ||||||||||||
Inj. Vol.: | 2 µL | ||||||||||||
Mobile Phase | |||||||||||||
A: | Water, 5 mM ammonium formate, 0.1% formic acid | ||||||||||||
B: | Acetonitrile, 0.1% formic acid | ||||||||||||
|
Detector | UV/Vis @ 228 nm |
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Instrument | UHPLC |
Sample Preparation | 2 g of chocolate was weighed into a 50 mL centrifuge tube. The sample was brought to a total volume of 40 mL with IPA. The sample was then sonicated at 40 °C for 5 minutes followed by gentle mixing by hand. This was repeated for an additional two cycles. The sample was stored in a -20 °C freezer for 30 minutes to allow the lipids to precipitate. The sample was then vortexed for 5 seconds followed by centrifugation at 3000 rpm for 5 minutes. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis. |
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