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|Column||Raptor ARC-18 (cat.# 931421E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||1.8 µm|
|Pore Size:||90 Å|
|Guard Column:||UltraShield UHPLC precolumn filter (cat.# 25809)|
|Inj. Vol.:||2 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Sample Preparation||2 g of chocolate was weighed into a 50 mL centrifuge tube. The sample was brought to a total volume of 40 mL with IPA. The sample was then sonicated at 40 °C for 5 minutes followed by gentle mixing by hand. This was repeated for an additional two cycles. The sample was stored in a -20 °C freezer for 30 minutes to allow the lipids to precipitate. The sample was then vortexed for 5 seconds followed by centrifugation at 3000 rpm for 5 minutes. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.|