Cannabis Chocolate Sample on Raptor ARC-18 1.8 μm by UHPLC-UV

	Peaks	t_R (min)
1.	Δ9-Tetrahydrocannabinol (Δ9-THC)	2.49

Column

Raptor ARC-18 (cat.# 931421E)

Dimensions:

100 mm x 3.0 mm ID

Particle Size:

1.8 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter (cat.# 25809)

Temp.:

30 °C

Standard/Sample

Diluent:

25:75 Water:methanol

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.0	25	75
4.00	1.0	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC
Sample Preparation	2 g of chocolate was weighed into a 50 mL centrifuge tube. The sample was brought to a total volume of 40 mL with IPA. The sample was then sonicated at 40 °C for 5 minutes followed by gentle mixing by hand. This was repeated for an additional two cycles. The sample was stored in a -20 °C freezer for 30 minutes to allow the lipids to precipitate. The sample was then vortexed for 5 seconds followed by centrifugation at 3000 rpm for 5 minutes. The supernatant was diluted 10-fold in 25:75 water:methanol, vortexed briefly, and filtered using a 0.2 μm Thomson SINGLE StEP standard filter vial (cat.#: 25893) prior to analysis.

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