Solvent Savings Analysis of 19 Cannabinoids on Raptor ARC-18 (2.7 μm by LC-UV)

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	1.570
2.	Cannabidivarin (CBDV)	1.728
3.	Cannabidiolic acid (CBDA)	2.159
4.	Cannabigerolic acid (CBGA)	2.304
5.	Cannabigerol (CBG)	2.411
6.	Cannabidiol (CBD)	2.537
7.	Tetrahydrocannabivarin (THCV)	2.770
8.	Cannabichromevarin (CBCV)	3.355
9.	Tetrahydrocannabivarinic acid (THCVA)	3.507

	Peaks	t_R (min)
10.	Cannabinol (CBN)	3.756
11.	Cannabinolic acid (CBNA)	4.471
12.	Δ9-Tetrahydrocannabinol (Δ9-THC)	4.706
13.	Δ8-Tetrahydrocannabinol (Δ8-THC)	4.865
14.	Cannabicyclol (CBL)	5.594
15.	Cannabichromene (CBC)	5.878
16.	Tetrahydrocannabinolic acid A (THCA-A)	6.213
17.	Cannabichromenic acid (CBCA)	6.965
18.	Cannabicyclolic acid (CBLA)	7.370
19.	Cannabicitran (CBT)	9.359

Column

Raptor ARC-18 (cat.# 9314A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge, 2.7 µm (cat.# 9314A0252)

Temp.:

30 °C

Standard/Sample

Cannabidivarin (CBDV) (cat.# 34123)

Cannabidiolic acid (CBDA) (cat.# 34094)

Cannabigerolic acid (CBGA) (cat.# 34112)

Cannabigerol (CBG) (cat.# 34091)

Cannabidiol (CBD) (cat.# 34011)

Tetrahydrocannabivarin (THCV) (cat.# 34100)

Cannabinol (CBN) (cat.# 34010)

d9-Tetrahydrocannabinol (d9-THC) (cat.# 34067)

d8-Tetrahydrocannabinol (d8-THC) (cat.# 34090)

Cannabichromene (CBC) (cat.# 34092)

d9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34093)

Cannabicyclol (CBL) (cat.# 34130)

Compounds not present in these mixes were obtain separately.

Diluent:

Methanol

Conc.:

50 ppm

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	25	75
10.00	0.4	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC

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