Cannabinoids in Gummy Edibles on Raptor ARC-18

	Peaks	t_R (min)	mg/g*
1.	Cannabidiolic acid (CBDA)	2.142	0.2
2.	Cannabigerol (CBG)	2.405	0.2
3.	Cannabidiol (CBD)	2.535	0.2
4.	Cannabinol (CBN)	3.776	0.2
5.	Δ9-Tetrahydrocannabinol (Δ9-THC)	4.753	0.2
6.	Tetrahydrocannabinolic acid A (THCA-A)	6.279	0.2

*Extract from a gummy sample initially spiked at 0.2 mg/g.

Column

Raptor ARC-18 (cat.# 9314A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 EXP guard column cartridge 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

30 °C

Standard/Sample

Cannabinoids standard (cat.# 34014)

Cannabigerol (cat.# 34091)

d9-Tetrahydrocannabinol (cat.# 34067)

d9-Tetrahydrocannabinolic acid A (cat.# 34111)

Diluent:

75:25 Acetonitrile:water

Conc.:

Expected concentration of 4 ppm in final extract from gummy initially spiked at 0.2 mg/g.

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	25	75
10.00	0.4	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC
Notes	Gummies were manually chopped into small pieces, and 1 g of sample was weighed in a 50 mL polypropylene tube. The sample was mixed with 5 mL of water and then vigorously vortexed until all gummy pieces were fully solubilized. The sample was fortified with cannabinoids at 0.2 mg/g. The spiked sample was further vortexed for 30 sec. 5 mL of acetonitrile acidified with 1% acetic acid was added to the sample, and this was followed by 30 sec vortex agitation. Then, a pouch of European EN 15662 QuEChERS extraction salts (cat.# 25849) was added to the sample. The sample was vortexed for 30 sec and then centrifuged for 5 min. 100 µL of organic extract was mixed with 900 µL of 75:25 acetonitrile:water. 2 µL of final extract was injected into the HPLC-UV system.

Download PDF