Standard Analysis of 21 Cannabinoids on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	1.775
2.	Cannabidivarin (CBDV)	1.973
3.	Cannabidiolic acid (CBDA)	2.442
4.	Cannabigerolic acid (CBGA)	2.575
5.	Cannabigerol (CBG)	2.732
6.	Cannabidiol (CBD)	2.904
7.	Tetrahydrocannabivarin (THCV)	3.203
8.	Cannabichromevarin (CBCV)	3.867
9.	Tetrahydrocannabivarinic acid (THCVA)	4.023
10.	Cannabinol (CBN)	4.321

	Peaks	t_R (min)
11.	Cannabidiphorol (CBDP)	4.866
12.	Cannabinolic acid (CBNA)	5.099
13.	Δ9-Tetrahydrocannabinol (Δ9-THC)	5.460
14.	Δ8-Tetrahydrocannabinol (Δ8-THC)	5.637
15.	Cannabicyclol (CBL)	6.496
16.	Cannabichromene (CBC)	6.788
17.	Tetrahydrocannabinolic acid A (THCA-A)	7.122
18.	Cannabichromenic acid (CBCA)	7.926
19.	Cannabicyclolic acid (CBLA)	8.458
20.	Tetrahydrocannabiphorol (THCP)	10.251
21.	Cannabicitran (CBT)	11.015

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Cannabidivarinic acid (CBDVA) (cat.# 34134)

Cannabidivarin (CBDV) (cat.# 34123)

Cannabidiolic acid (CBDA) (cat.# 34094)

Cannabigerolic acid (CBGA) (cat.# 34135)

Cannabigerol (CBG) (cat.# 34091)

Cannabidiol (CBD) (cat.# 34011)

Tetrahydrocannabivarin (THCV) (cat.# 34100)

Cannabinol (CBN) (cat.# 34010)

d9-Tetrahydrocannabinol (d9-THC) (cat.# 34067)

d8-Tetrahydrocannabinol (d8-THC) (cat.# 34090)

Cannabicyclol (CBL) (cat.# 34130)

Cannabichromene (CBC) (cat.# 34092)

d9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34111)

Compounds not present in these mixes were obtained separately.

Diluent:

Methanol

Conc.:

50 ppm

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
12	1.5	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC

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