UHPLC Analysis of 21 Cannabinoids on Raptor ARC-18 1.8 μm by LC-UV

	Peaks	t_R (min)
1.	Cannabidivarinic acid (CBDVA)	0.813
2.	Cannabidivarin (CBDV)	0.888
3.	Cannabidiolic acid (CBDA)	1.102
4.	Cannabigerolic acid (CBGA)	1.172
5.	Cannabigerol (CBG)	1.222
6.	Cannabidiol (CBD)	1.278
7.	Tetrahydrocannabivarin (THCV)	1.398
8.	Cannabichromevarin (CBCV)	1.679
9.	Tetrahydrocannabivarinic acid (THCVA)	1.768
10.	Cannabinol (CBN)	1.872

	Peaks	t_R (min)
11.	Cannabidiphorol (CBDP)	2.088
12.	Cannabinolic acid (CBNA)	2.222
13.	Δ9-Tetrahydrocannabinol (Δ9-THC)	2.325
14.	Δ8-Tetrahydrocannabinol (Δ8-THC)	2.394
15.	Cannabicyclol (CBL)	2.740
16.	Cannabichromene (CBC)	2.882
17.	Tetrahydrocannabinolic acid A (THCA-A)	3.065
18.	Cannabichromenic acid (CBCA)	3.418
19.	Cannabicyclolic acid (CBLA)	3.620
20.	Tetrahydrocannabiphorol (THCP)	4.273
21.	Cannabicitran (CBT)	4.552

Column

Raptor ARC-18 (cat.# 931421E)

Dimensions:

100 mm x 3.0 mm ID

Particle Size:

1.8 µm

Pore Size:

90 Å

Guard Column:

UltraShield UHPLC precolumn filter, 0.2 μm frit (cat.# 25809)

Temp.:

30 °C

Standard/Sample

Cannabidivarinic acid (CBDVA) (cat.# 34134)

Cannabidivarin (CBDV) (cat.# 34123)

Cannabidiolic acid (CBDA) (cat.# 34094)

Cannabigerolic acid (CBGA) (cat.# 34135)

Cannabigerol (CBG) (cat.# 34091)

Cannabidiol (CBD) (cat.# 34011)

Tetrahydrocannabivarin (THCV) (cat.# 34100)

Cannabinol (CBN) (cat.# 34010)

d9-Tetrahydrocannabinol (d9-THC) (cat.# 34067)

d8-Tetrahydrocannabinol (d8-THC) (cat.# 34090)

Cannabicyclol (CBL) (cat.# 34130)

Cannabichromene (CBC) (cat.# 34092)

d9-Tetrahydrocannabinolic acid A (THCA-A) (cat.# 34111)

Compounds not present in these mixes were obtained separately.

Diluent:

Methanol

Conc.:

50 ppm

Inj. Vol.:

1 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1	25	75
5	1	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC

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