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|Peaks||tR (min)||Conc. (µg/mL)*|
|1.||Cannabidiolic acid (CBDA)||2.18||3.33|
|6.||Tetrahydrocannabinolic acid A (THCA-A)||6.42||3.33|
|Column||Raptor ARC-18 (cat.# 9314A62)|
|Dimensions:||150 mm x 2.1 mm ID|
|Particle Size:||2.7 µm|
|Pore Size:||90 Å|
|Guard Column:||Raptor ARC-18 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)|
|Standard/Sample||Cannabinoids standard (cat.# 34014)|
|Cannabigerol (cat.# 34091)|
|d9-Tetrahydrocannabinol (cat.# 34067)|
|d9-Tetrahydrocannabinolic acid A (cat.# 34111)|
|Conc.:||Expected concentration of 3.33 ppm in final extract from chocolate initially spiked at 0.2 mg/g.|
|Inj. Vol.:||2 µL|
|A:||Water, 5 mM ammonium formate, 0.1% formic acid|
|B:||Acetonitrile, 0.1% formic acid|
|Detector||UV/Vis @ 228 nm|
|Sample Preparation||Chocolate was pulverized using a SPEX Freezer/Mill grinder, and a 0.5 g sample was fortified with cannabinoids at 0.2 mg/g. Then, 0.5 mL of isopropyl alcohol was added to the sample. The sample was vortexed for 10 sec or until a homogenous mixture was obtained. Afterwards, 2.5 mL of acetonitrile acidified with acetic acid at 1% v/v was added to the vial. Once again, the mixture was vortexed for 30 sec, and then centrifuged for 5 min at 4300 ×g at room temperature. 100 µL of chocolate extract was mixed with 900 µL of 75:25 acetonitrile:water, and the final mix was centrifuged for 10 min at 4 ⁰C. 2 µL of final extract was injected into the HPLC-UV system.|
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