Solvent-Savings Analysis of Cannabinoids Acids 7 Standard on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Cannabidivarinic acid (CBDVA)	1.50	50
2.	Cannabidiolic acid (CBDA)	2.06	50
3.	Cannabigerolic acid (CBGA)	2.20	50
4.	Tetrahydrocannabivarinic acid (THCVA)	3.35	50
5.	Cannabinolic acid (CBNA)	4.30	50
6.	Tetrahydrocannabinolic acid A (THCA-A)	5.93	50
7.	Cannabichromenic acid (CBCA)	6.68	50

Column

Raptor ARC-18 (cat.# 9314A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

30 °C

Standard/Sample

Cannabinoids acids 7 standard (cat.# 34144)

Diluent:

Acetonitrile

Conc.:

50 ppm

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	25	75
10.00	0.4	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC
Sample Preparation	Standards were aliquoted into 2 mL, screw-thread vials (cat.# 21143) and capped with short-cap, screw-vial closures (cat.# 24498).

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