Solvent-Savings Analysis of Cannabinoid Acids and Neutrals Standards on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Cannabidivarinic acid (CBDVA)	1.500	50
2.	Cannabidivarin (CBDV)	1.650	50
3.	Cannabidiolic acid (CBDA)	2.060	50
4.	Cannabigerolic acid (CBGA)	2.195	50
5.	Cannabigerol (CBG)	2.305	50
6.	Cannabidiol (CBD)	2.420	50
7.	Tetrahydrocannabivarin (THCV)	2.635	50
8.	Tetrahydrocannabivarinic acid (THCVA)	3.330	50

	Peaks	t_R (min)	Conc. (µg/mL)
9.	Cannabinol (CBN)	3.570	50
10.	Cannabinolic acid (CBNA)	4.270	50
11.	Delta-9-Tetrahydrocannabinol (Δ9-THC)	4.480	50
12.	Delta-8-Tetrahydrocannabinol (Δ8-THC)	4.630	50
13.	Cannabicyclol (CBL)	5.320	50
14.	Cannabichromene (CBC)	5.605	50
15.	Tetrahydrocannabinolic acid A (THCA-A)	5.890	50
16.	Cannabichromenic acid (CBCA)	6.650	50

Column

Raptor ARC-18 (cat.# 9314A62)

Dimensions:

150 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 5 mm, 2.1 mm ID, 2.7 µm (cat.# 9314A0252)

Temp.:

30 °C

Standard/Sample

Cannabinoids acids 7 standard (cat.# 34144)

Cannabinoids neutrals 9 standard (cat.# 34132)

Diluent:

Acetonitrile

Conc.:

50 ppm

Inj. Vol.:

2 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	25	75
10.00	0.4	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC
Sample Preparation	Standards were aliquoted into 2 mL, screw-thread vials (cat.# 21143) and capped with short-cap, screw-vial closures (cat.# 24498).

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