Cannabinoids Acids 7 Standard on Raptor ARC-18 2.7 μm by LC-UV

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Cannabidivarinic acid (CBDVA)	1.860	50
2.	Cannabidiolic acid (CBDA)	2.610	50
3.	Cannabigerolic acid (CBGA)	2.785	50
4.	Tetrahydrocannabivarinic acid (THCVA)	4.325	50
5.	Cannabinolic acid (CBNA)	5.550	50
6.	Tetrahydrocannabinolic acid A (THCA-A)	7.810	50
7.	Cannabichromenic acid (CBCA)	8.780	50

Column

Raptor ARC-18 (cat.# 9314A65)

Dimensions:

150 mm x 4.6 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Guard Column:

Raptor ARC-18 5 mm, 4.6 mm ID, 2.7 µm (cat.# 9314A0250)

Temp.:

30 °C

Standard/Sample

Cannabinoids acids 7 standard (cat.# 34144)

Diluent:

Acetonitrile

Conc.:

50 ppm

Inj. Vol.:

5 µL

Mobile Phase

Water, 5 mM ammonium formate, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	1.5	25	75
10.00	1.5	25	75

Detector	UV/Vis @ 228 nm
Instrument	UHPLC
Sample Preparation	Standards were aliquoted into 2 mL, screw-thread vials (cat.# 21143) and capped with short-cap, screw-vial closures (cat.# 24498).

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