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|1.||N,N-desmethyl Psilocybin (Norbaeocystin)||1.314|
|2.||N-desmethyl Psilocybin (Baeocystin)||1.750|
|Column||Force Biphenyl (cat.# 962931E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||3 µm|
|Pore Size:||100 Å|
|Guard Column:||Force Biphenyl EXP Guard Cartridge 5 mm, 3.0 mm ID (cat.# 962950253)|
|Inj. Vol.:||1 µL|
|A:||Water, 10 mM ammonium formate, 0.1% formic acid|
|B:||Methanol, 0.1% formic acid|
|Detector||UV/Vis @ 222 nm|
|Flow Cell Size:||500 nL|
|Instrument||Waters ACQUITY UPLC H-Class|
|Sample Preparation||Standards were prepared in a 2.0 mL amber short-cap vial (cat.# 21142) with a 250 µL glass insert (cat.# 24518) and capped with a 9 mm short cap (cat.# 24497).|
|Notes||System and column were passivated using LC Passivation Solution (cat.# 32475). The outlined method conditions were used to perform five injections of passivation solution (5 µL) on column, diverting to waste. Five warm-up injections were completed prior to analysis. |
Storage Conditions: Flush column using acetonitrile with 0.1% formic acid for 5 min.
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