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|1.||N,N-desmethyl Psilocybin (Norbaeocystin)||1.585|
|Column||Force Biphenyl (cat.# 962931E)|
|Dimensions:||100 mm x 3.0 mm ID|
|Particle Size:||3.0 µm|
|Pore Size:||100 Å|
|Guard Column:||Force Biphenyl EXP Guard Cartridge 5.0 mm, 3.0 mm ID (cat.# 962950253)|
|Inj. Vol.:||3 µL|
|A:||Water,10 mM ammonium formate, 0.1% formic acid|
|B:||Methanol, 0.1% formic acid|
|Detector||UV/Vis @ 222 nm|
|Sample Preparation||1 g of freeze-dried, homogenized chocolate was weighed into a 50 mL Environmental Express tube (6.0 µm) followed by the addition of 2 mL of isopropanol and 1 min of vortexing. 18 mL of water with 1% acetic acid was added, tube capped, and vortexed for 5 min at 2500 RPM. The sample was then filtered with a plunger. An aliquot was obtained using a 3 mL syringe (cat.#22773) equipped with a 0.22 µm PTFE syringe filter (cat.# 23984). The sample was then diluted 5x with HPLC grade water into a 2.0 mL amber, short-cap vial (cat.# 21142) and capped with a 9 mm short cap (cat.#24497).|
|Notes||The guard cartridge was equipped with an UltraShield precolumn filter, 0.22 µm (cat.# 25809).|
System and column were passivated using LC Passivation Solution (cat.# 32475). The outlined method conditions were used to perform five injections of passivation solution (5 µL) on column, diverting to waste. Five warm-up injections were completed prior to analysis.
Storage Conditions: Flush column using acetonitrile with 0.1% formic acid for 5 min.
|Acknowledgement||We would like to thank 2 River Labs for their use and accommodations of lab space, instruments, and samples during collaboration.|
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