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Analysis of Psychoactive Alkaloids in Infused Gummy Edibles by HPLC-UV

ColumnForce Biphenyl (cat.# 962931E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:3.0 µm
Pore Size:100 Å
Guard Column:Force Biphenyl EXP Guard Cartridge 5.0 mm, 3.0 mm ID (cat.# 962950253)
Temp.:50 °C
Inj. Vol.:3 µL
Mobile Phase
A:Water, 10 mM ammonium formate, 0.1% formic acid
B:Methanol, 0.1% formic acid
Time (min)Flow (mL/min)%A%B
DetectorUV/Vis @ 222 nm
InstrumentAgilent 1100
Sample Preparation1 g of freeze-dried, homogenized gummy was weighed into a 50 mL Environmental Express tube (6.0 µm). 20 mL of acidified water with 1% acetic acid was added, tube capped, and vortexed for 5 min at 2500 RPM. The sample was then filtered with a plunger. An aliquot was obtained using a 3 mL syringe (cat.#22773) equipped with a 0.22 µm PTFE syringe filter (cat.# 23984). The sample was then diluted 5x with HPLC grade water into a 2.0 mL amber, short-cap vial (cat.# 21142) and capped with a 9 mm short cap (cat.#24497).

NotesThe guard cartridge was equipped with an UltraShield precolumn filter, 0.22 µm (cat.# 25809).

System and column were passivated using LC Passivation Solution (cat.# 32475).
The outlined method conditions were used to perform five injections of passivation solution (5 µL) on column, diverting to waste. Five warm-up injections were completed prior to analysis.

Storage Conditions: Flush column using acetonitrile with 0.1% formic acid for 5 min.

AcknowledgementWe would like to thank 2 River Labs for their use and accommodations of lab space, instruments, and samples during collaboration.