Chlormequat, Mepiquat, Paraquat, and Diquat on Raptor HILIC-Si by LC-MS/MS

	Peaks	t_R (min)	Precursor Ion 1	Product Ion 1	Precursor Ion 2	Product Ion 2
1.	Chlormequat	1.325	122.0	58.2	122.0	63.1
2.	Mepiquat Chloride	1.724	114.0	98.2	114.0	58.2
3.	Paraquat	6.036	186.0	171.0	171.0	77.2
4.	Diquat	6.491	183.0	157.0	183.0	130.0

Column

Raptor HILIC-Si (cat.# 9310A52)

Dimensions:

50 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

60 °C

Standard/Sample

Paraquat & Diquat Calibration Mix (cat.# 32437)

Compounds not present in these mixes were obtained separately.

Diluent:

Acetonitrile:water 90:10, 5 mM ammonium formate, 0.5% formic acid

Conc.:

100 ng/mL

Inj. Vol.:

1 µL

Mobile Phase

Water, 50 mM ammonium formate, 0.5% formic acid

Acetonitrile:water 90:10, 5 mM ammonium formate, 0.5% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.6	0	100
4.00	0.6	25	75
7.00	0.6	25	75
7.01	0.6	0	100
10.00	0.6	0	100

Detector	Shimadzu 8060 MS/MS
Ion Source:	Electrospray
Ion Mode:	ESI+
Mode:	MRM
Instrument	Shimadzu Nexera X2
Sample Preparation	Samples were aliquoted into a 2.0 mL, 9 mm Screw-Thread Polypropylene Vials (cat.# 23242) and capped with 9 mm Solid-Top Polyethylene Caps (cat.# 23244).

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