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Emtricitabine and Tenofovir on Ultra IBD by LC-MS/MS

LC_PH0530
PeakstR (min)MRM 1MRM 2
1.Emtricitabine1.03248.1/130.2248.1/113.1
2.Tenofovir1.28288.1/176.3288.1/159.2
ColumnUltra IBD (cat.# 9175552)
Dimensions:50 mm x 2.1 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:40 °C
Sample
Diluent:Water
Conc.:100 ng/mL
Inj. Vol.:10 µL
Mobile PhaseWater + 0.1% formic acid:Methanol + 0.1% formic acid (85:15)
Flow:0.3 mL/min
DetectorABSCIEX API 4000
Ion Source:TurboIonSpray®
Ion Mode:ESI+
Ion Spray Voltage:5.0 kV
Curtain Gas:40 psi (275.8 kPa)
Gas 1:45 psi (310.3 kPa)
Gas 2:40 psi (275.8 kPa)
CAD:12 psi (82.7 kPa)
Source Temp.:450 °C
Mode:MRM
Dwell Time:150 ms
InstrumentApplied Biosystems/MDS Sciex LC-MS/MS System