Emtricitabine and Tenofovir on Ultra IBD by LC-MS/MS

	Peaks	t_R (min)	MRM 1	MRM 2
1.	Emtricitabine	1.03	248.1/130.2	248.1/113.1
2.	Tenofovir	1.28	288.1/176.3	288.1/159.2

Standard/Sample
Column	Ultra IBD (cat.# 9175552)
Dimensions:	50 mm x 2.1 mm ID
Particle Size:	5 µm
Pore Size:	100 Å

Temp.:	40 °C
Diluent:	Water
Conc.:	100 ng/mL
Inj. Vol.:	10 µL
Mobile Phase	Water + 0.1% formic acid:Methanol + 0.1% formic acid (85:15)
Flow:	0.3 mL/min

Detector	ABSCIEX API 4000
Ion Source:	TurboIonSpray^®
Ion Mode:	ESI+
Ion Spray Voltage:	5.0 kV
Curtain Gas:	40 psi (275.8 kPa)
Gas 1:	45 psi (310.3 kPa)
Gas 2:	40 psi (275.8 kPa)
CAD:	12 psi (82.7 kPa)
Source Temp.:	450 °C
Mode:	MRM
Dwell Time:	150 ms
Instrument	Applied Biosystems/MDS Sciex LC-MS/MS System

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