Weight Loss Drugs on Force Biphenyl

	Peaks	t_R (min)	Positive MRM	Negative MRM
1.	Phentermine	1.78	150.1/133.2	-
2.	Topiramate	2.88	-	338.1/78.0

Column

Force Biphenyl (cat.# 9629312)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

3 µm

Pore Size:

100 Å

Temp.:

30 °C

Standard/Sample

Diluent:

Water:acetonitrile (90:10)

Conc.:

50 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water, 0.1% formic acid

Acetonitrile, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.5	90	10
4.00	0.5	10	90
4.50	0.5	10	90
4.60	0.5	90	10
6.00	0.5	90	10

Detector	MS/MS
Mode:	MRM
Instrument	UHPLC
Notes	The detector is operated in multi-period mode. Period 1, from 0.0 to 2.4 min, is ESI+ mode, and Period 2, from 2.4 to 6.0 min, is ESI- mode.

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