Nitrosamines on Raptor Biphenyl

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
1.	N-Nitrosodiethanolamine (NDELA)	0.90	10	135.1	74.0
2.	N-Nitrosodimethylamine (NDMA)	1.52	10	75.1	58.0
3.	N-Nitrosodiisobutylamine (NDIPLA)	1.90	10	163.0	88.0
4.	N-Nitrosomorpholine (NMOR)	2.38	10	117.1	45.2
5.	N-Nitrosomethylethylamine (NMEA)	2.57	10	89.1	61.0
6.	N-Nitroso-N-methyl-4-aminobutyric acid (NMBA)	2.61	10	147.1	117.0
7.	N-Nitrosopyrrolidine (NPYR)	3.12	10	101.1	55.1
8.	N-Nitrosodiethylamine (NDEA)	3.86	10	103.1	75.0

	Peaks	t_R (min)	Conc. (ng/mL)	Precursor Ion	Product Ion
9.	N-Nitrosopiperidine (NPIP)	4.66	10	115.1	69.0
10.	N-Nitrosoethylisopropylamine (NEIPA)	4.91	10	117.1	75.0
11.	N-Nitrosodiisopropylamine (NDIPA)	5.79	10	131.1	43.0
12.	N-Nitrosodipropylamine (NDPA)	6.07	10	131.1	43.0
13.	N-Nitrosomethylphenylamine (NMPA)	6.30	10	137.0	65.9
14.	N-Nitrosodiisobutylamine (NDIBA)	7.23	10	159.1	57.1
15.	N-Nitrosodibutylamine (NDBA)	7.46	10	159.1	57.1
16.	N-Nitrosodiphenylamine (NDPHA)	8.17	10	199.1	169.0

Column

Raptor Biphenyl (cat.# 9309A12)

Dimensions:

100 mm x 2.1 mm ID

Particle Size:

2.7 µm

Pore Size:

90 Å

Temp.:

40 °C

Standard/Sample

Nitrosamine calibration mix, Method 521 (cat.# 31898)

N-Nitrosodiphenylamine (cat.# 31429)

Diluent:

Water

Conc.:

10 ng/mL

Inj. Vol.:

5 µL

Mobile Phase

Water, 0.1% formic acid

Methanol, 0.1% formic acid

Time (min)	Flow (mL/min)	%A	%B
0.00	0.4	95	5
9.00	0.4	5	95
9.01	0.4	95	5
11.00	0.4	95	5

Detector	MS/MS
Ion Source:	APCI
Ion Mode:	APCI+
Mode:	MRM
Instrument	UHPLC
Sample Preparation	Standard solutions were prepared in 2 mL, screw-thread amber vials (cat.# 21143) and capped with 9 mm, short-cap, screw-vial closures (cat.# 24498).

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