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Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab

Conventional HPLC columns built for solid performance, exceptional value, and compact storage.

  • Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
  • Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
  • Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
  • Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods.
  • Newly designed minimal packaging for compact storage—download serial #-specific certificates of analysis at restek.com/documentation

 

Roc Family of HPLC Phases

 

C18

C8

Phenyl-Hexyl

Cyano

Silica

USP Phase Code

L1

L7

L11

L10

L3

Stationary Phase Category

C18, octadecylsilane

C8, octylsilane

phenyl

cyano

bare silica

Ligand Type

monomeric C18

monomeric C8

phenyl-hexyl

cyanopropyl

n/a

Particle Size

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

3 μm or 5 μm, spherical

Pore Size

100 Å

100 Å

100 Å

100 Å

100 Å

Surface Area

300 m2/g

300 m2/g

300 m2/g

300 m2/g

300 m2/g

Carbon Load

20%

12%

15%

8%

n/a

End-Cap

yes

yes

yes

yes

n/a

pH Range

2.5 to 8.0

2.5 to 8.0

2.0 to 8.0

2.5 to 8.0

2.5 to 8.0

Maximum Temperature

80 °C

80 °C

80 °C

80 °C

80 °C

 

Figure 1: Over 1,000 injections and Roc HPLC columns will still offer reliable performance, regardless of which dimension your method calls for.

Figure 2: Add a Roc HPLC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency.

LC_GN0565
PeaksConc.
(mg/mL)
1.Uracil0.02
2.Toluene3.00
3.Naphthalene0.50
4.Biphenyl0.06
ColumnRoc C8 (cat.# 9533565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Guard Column:Roc C8 10 mm, 4.0 mm ID, 5 µm (cat.# 953350210)
Temp.:30 °C
Standard/SampleCustom mix
Diluent:50:50 Acetonitrile:water
Inj. Vol.:2 µL
Mobile Phase
A:Water
B:Acetonitrile
Time (min)Flow (mL/min)%A%B
0.001.03070
8.001.03070
DetectorUV @ 254 nm
InstrumentHPLC

Figure 3: From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again.

Reversed Phase

LC_GN0562
ColumnRoc C18 (cat.# 9534565)
Dimensions:150 mm x 4.6 mm ID
Particle Size:5 µm
Pore Size:100 Å
Temp.:35 °C
Inj. Vol.:5 µL
Mobile Phase0.05% Formic acid in water:0.05% Formic acid in acetonitrile (40:60)
Flow:1.0 mL/min
DetectorUV/Vis @ 220 nm
Cell Temp:35 °C
InstrumentHPLC

 

Normal Phase

LC_GN0563
PeakstR (min)
1.Benzene3.5
2.Nitrobenzene4.1
3.1,3,5-Trinitrobenzene5.5
4.2,4-Dinitrotoluene7.4
ColumnRoc Cyano (cat.# 953631E)
Dimensions:100 mm x 3.0 mm ID
Particle Size:3 µm
Pore Size:100 Å
Temp.:40 °C
Inj. Vol.:1 µL
Mobile PhaseWater:acetonitrile (80:20)
Flow:0.6 mL/min
DetectorUV/Vis @ 254 nm
Cell Temp:40 °C
InstrumentHPLC

Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors

Columns

Analyte

tR

Area

Height

USP Tailing

Asymmetry (10%)

k'

Plate Number

Resolution

Roc

Ethylparaben

3.252

1,407,609

281,358

1.176

1.148

1.182

9,738

12.5

Ethinyl Estradiol

5.410

1,869,684

234,346

1.075

1.064

2.631

10,264

Vendor A

Ethylparaben

3.060

1,424,621

215,698

1.907

1.608

1.013

6,732

10.1

Ethinyl Estradiol

4.889

1,860,659

223,879

1.311

1.217

2.217

8,575

Vendor B

Ethylparaben

2.873

1,426,068

319,850

1.143

1.124

0.866

9,706

9.9

Ethinyl Estradiol

4.307

1,867,049

287,414

1.064

1.057

1.797

10,031

Vendor C

Ethylparaben

2.763

1,413,099

315,080

1.262

1.217

0.932

8,643

11.9

Ethinyl Estradiol

4.580

1,868,724

270,662

1.143

1.122

2.203

9,700

Vendor D

Ethylparaben

3.157

2,939,187

357,541

1.159

0.965

1.084

4,478

12.2

Ethinyl Estradiol

5.816

1,771,785

182,056

1.301

1.232

2.839

8,954

Vendor E

Ethylparaben

3.873

1,498,446

244,642

1.172

1.134

1.221

9,348

13.7

Ethinyl Estradiol

6.839

1,781,453

174,955

1.080

1.063

2.921

9,963

 

Figure 4: Count on Roc HPLC columns from Restek for outstanding chromatographic performance.

Roc HPLC Column

LC_EX0518
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.25250
2.Ethinyl estradiol5.410200

 

Vendor A

LC_EX0521
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.06050
2.Ethinyl estradiol4.889200

 

Vendor B

LC_EX0520
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben2.87350
2.Ethinyl estradiol4.307200

 

Vendor C

LC_EX0519
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben2.76350
2.Ethinyl estradiol4.580200

 

Vendor D

LC_EX0522
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.15750
2.Ethinyl estradiol5.816200

 

Vendor E

LC_EX0523
PeakstR (min)Conc.
(µg/mL)
1.Ethylparaben3.87350
2.Ethinyl estradiol6.839200

Chromatographic conditions for Table I and Figure 4
All columns are C18 stationary phase (5 µm, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL/min; Injection volume: 25 µL; Instrument: HPLC with UV detector, 280 nm; Sample: 200 µg/mL ethinyl estradiol and 50 µg/mL ethylparaben in water:acetonitrile (1:1).

Get more of what you want, and less of what you don't, with Roc HPLC columns from Restek.

GNSS2332B-UNV

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