Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab

Conventional HPLC columns built for solid performance, exceptional value, and compact storage.

Share:
Download PDF

Conventional HPLC column built to be the cornerstone for your lab; pressure rated for any 400 bar HPLC system.
Solid and reliable—delivers the peak shape, reproducibility, ruggedness, and performance you demand.
Exceptional value for routine analyses and large-volume workflows—high-purity, fully porous silica backed by quality manufacturing features a variety of phases to fit most HPLC methods.
Ideal for a wide range of applications from food to pharma; 5 phases cover 98% of USP methods.
Newly designed minimal packaging for compact storage—download serial #-specific certificates of analysis at restek.com/documentation

Roc Family of HPLC Phases

	C18	C8	Phenyl-Hexyl	Cyano	Silica
USP Phase Code	L1	L7	L11	L10	L3
Stationary Phase Category	C18, octadecylsilane	C8, octylsilane	phenyl	cyano	bare silica
Ligand Type	monomeric C18	monomeric C8	phenyl-hexyl	cyanopropyl	n/a
Particle Size	3 μm or 5 μm, spherical	3 μm or 5 μm, spherical	3 μm or 5 μm, spherical	3 μm or 5 μm, spherical	3 μm or 5 μm, spherical
Pore Size	100 Å	100 Å	100 Å	100 Å	100 Å
Surface Area	300 m²/g	300 m²/g	300 m²/g	300 m²/g	300 m²/g
Carbon Load	20%	12%	15%	8%	n/a
End-Cap	yes	yes	yes	yes	n/a
pH Range	2.5 to 8.0	2.5 to 8.0	2.0 to 8.0	2.5 to 8.0	2.5 to 8.0
Maximum Temperature	80 °C	80 °C	80 °C	80 °C	80 °C

**Figure 1:** Over 1,000 injections and Roc HPLC columns will still offer reliable performance, regardless of which dimension your method calls for.

**Figure 2:** Add a Roc HPLC guard to further extend the life of your analytical column without impacting your retention, peak shape, or efficiency.

	Peaks	Conc. (mg/mL)
1.	Uracil	0.02
2.	Toluene	3.00
3.	Naphthalene	0.50
4.	Biphenyl	0.06

Detector	UV @ 254 nm
Instrument	HPLC

**Figure 3:** From one column to the next, one lot to the next, Roc columns provide results you can trust time and time again.

	Peaks	t_R (min)
1.	p-Tolunitrile	3.7
2.	4-Butylbenzoic acid	5.2
3.	3,4-Dimethylanisole	7.3
4.	Mefenamic acid	8.1
5.	p-Xylene	9.7

Column	Roc C18 (cat.# 9534565)
Dimensions:	150 mm x 4.6 mm ID
Particle Size:	5 µm
Pore Size:	100 Å

Temp.:	35 °C
Inj. Vol.:	5 µL
Mobile Phase	0.05% Formic acid in water:0.05% Formic acid in acetonitrile (40:60)
Flow:	1.0 mL/min

Detector	UV/Vis @ 220 nm
Cell Temp:	35 °C
Instrument	HPLC

	Peaks	t_R (min)
1.	Benzene	3.5
2.	Nitrobenzene	4.1
3.	1,3,5-Trinitrobenzene	5.5
4.	2,4-Dinitrotoluene	7.4

Column	Roc Cyano (cat.# 953631E)
Dimensions:	100 mm x 3.0 mm ID
Particle Size:	3 µm
Pore Size:	100 Å

Temp.:	40 °C
Inj. Vol.:	1 µL
Mobile Phase	Water:acetonitrile (80:20)
Flow:	0.6 mL/min

Detector	UV/Vis @ 254 nm
Cell Temp:	40 °C
Instrument	HPLC

Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors

Columns	Analyte	tR	Area	Height	USP Tailing	Asymmetry (10%)	k'	Plate Number	Resolution
Roc	Ethylparaben	3.252	1,407,609	281,358	1.176	1.148	1.182	9,738	12.5
Roc	Ethinyl Estradiol	5.410	1,869,684	234,346	1.075	1.064	2.631	10,264	12.5
Vendor A	Ethylparaben	3.060	1,424,621	215,698	1.907	1.608	1.013	6,732	10.1
Vendor A	Ethinyl Estradiol	4.889	1,860,659	223,879	1.311	1.217	2.217	8,575	10.1
Vendor B	Ethylparaben	2.873	1,426,068	319,850	1.143	1.124	0.866	9,706	9.9
Vendor B	Ethinyl Estradiol	4.307	1,867,049	287,414	1.064	1.057	1.797	10,031	9.9
Vendor C	Ethylparaben	2.763	1,413,099	315,080	1.262	1.217	0.932	8,643	11.9
Vendor C	Ethinyl Estradiol	4.580	1,868,724	270,662	1.143	1.122	2.203	9,700	11.9
Vendor D	Ethylparaben	3.157	2,939,187	357,541	1.159	0.965	1.084	4,478	12.2
Vendor D	Ethinyl Estradiol	5.816	1,771,785	182,056	1.301	1.232	2.839	8,954	12.2
Vendor E	Ethylparaben	3.873	1,498,446	244,642	1.172	1.134	1.221	9,348	13.7
Vendor E	Ethinyl Estradiol	6.839	1,781,453	174,955	1.080	1.063	2.921	9,963	13.7

**Figure 4:** Count on Roc HPLC columns from Restek for outstanding chromatographic performance.

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	3.252	50
2.	Ethinyl estradiol	5.410	200

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	3.060	50
2.	Ethinyl estradiol	4.889	200

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	2.873	50
2.	Ethinyl estradiol	4.307	200

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	2.763	50
2.	Ethinyl estradiol	4.580	200

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	3.157	50
2.	Ethinyl estradiol	5.816	200

	Peaks	t_R (min)	Conc. (µg/mL)
1.	Ethylparaben	3.873	50
2.	Ethinyl estradiol	6.839	200

Chromatographic conditions for Table I and Figure 4
All columns are C18 stationary phase (5 µm, 150 x 4.6 mm). Conditions are as follows. Mobile phase: water:acetonitrile (1:1); Flow rate: 1 mL/min; Injection volume: 25 µL; Instrument: HPLC with UV detector, 280 nm; Sample: 200 µg/mL ethinyl estradiol and 50 µg/mL ethylparaben in water:acetonitrile (1:1).

Get more of what you want, and less of what you don't, with Roc HPLC columns from Restek.

GNSS2332B-UNV

Roc HPLC Columns: The Reliable Cornerstone of Your LC Lab

Conventional HPLC columns built for solid performance, exceptional value, and compact storage.

Roc Family of HPLC Phases

Roc HPLC Columns from Restek Perform Better Than or Equal To Columns from Other Vendors

Get more of what you want, and less of what you don't, with Roc HPLC columns from Restek.

Related Products

Roc C18 HPLC Columns (USP L1)

Roc C8 HPLC Columns (USP L7)

Roc Phenyl-Hexyl HPLC Columns (USP L11)

Roc Cyano HPLC Columns (USP L10)

Roc Silica HPLC Columns (USP L3)

Roc LC Guard Column Cartridges

Roc LC Guard Column Holder

Replacement PEEK Ferrules for Roc LC Guard Column Holder

Related Videos

Choosing Your LC Stationary Phase

Choosing Your LC Particle

Choosing Your LC Column Dimensions

Connecting Your LC Column

Related Articles

LC Method Translation: Will Switching Column Dimensions Speed Up Sample Analyses?

Restek LC Guard Column Selector

Diagnosing and Preventing High Back Pressure in LC Systems