Resource Hub / Video Library / Sonicating Your Reference Standard

Sonicating Your Reference Standard


Depending on how you store your reference standards, did you know it’s possible for some of the analytes to fall out of the solution? This will impact the concentration of your analytes, leading to problems with your analysis. 

In this Restek Tip well demonstrate how sonication can be used to help return these analytes to the solution. Well explore how to sonicate your ampuls and also share some tips on safety. 

Additional Resources

Restek Recommended Tools & Parts

 Connect with Us


Depending on the storage conditions of reference standards, they may need to be sonicated before use.

In today's Restek Tip, we're going to look at why you may need to sonicate your reference standards and how to do it. Let’s start with the why. If your standard is stored at low temperature, it's possible some of the analytes will fall out of solution. This will impact your analytes concentration, leading to problems with your analysis. In this case, sonication will be necessary to help ensure all analytes are in solution before transferring and using your standard.

To sonicate, first check that the sonicator is free from mineral buildup. Next, ensure there is enough deionized water in the bath—the water level may depend on your lab's procedures and equipment. Now place the ampul into the sonicator and set it to run for 10 to 15 minutes. The sonication process does generate heat, which can increase pressure within the ampul. We recommend that you allow the reference standard to cool to room temperature and to open the ampul within a hood to avoid potential splashing.

If you have any questions, please contact us at, or use the comments below. And, please remember to like and subscribe. Thank you very much for joining us for this Restek Tip.